Study On The Effect And Mechanism Of Allogeneic Platelet-Rich Plasma On Collagen Synthesis In Wounds
Keywords
Platelet-Rich Plasma, Wound Healing, Collagen, Synthesis Mechanism
Abstract
Abstract Objective To explore the effect and mechanism of allogeneic platelet-rich plasma (PRP) in promoting collagen synthesis in wounds. Methods: Fifty 7-week-old male SD clean-grade rats were used. 10 rats were used to prepare platelet-rich plasma. The remaining 40 rats were randomly divided into PRP group and control group, with 20 rats in each group. The wounds of rats in the PRP group were Apply 0.1 ml of allogeneic PRP, and apply the same dose of normal saline to the control group. The wound regeneration and healing conditions are observed. Masson staining is used to analyze cell and tissue morphological changes. Western blotting is used to detect the protein expression of type ¢ñ and type ¢ó collagen; Real-time quantitative PCR was used to detect the expression of type ¢ñ and type ¢ó collagen tissue mRNA. Comparison of measurement data was performed using t test, and comparison between groups was performed using one-way analysis of variance. Results On days 3, 6, 10 and 15 after wound formation, the wound healing rate in the PRP group was significantly higher than that in the control group (30.33¡À3.35 vs. 18.35¡À2.04, 55.51¡À2.74 vs. 36.83¡À2.34, 79.64¡À1.40 vs. 56.92¡À1.44, 86.88 ¡À2.12 vs. 65.80¡À1.76), the difference was statistically significant (t=13.66~50.48, P values all <0.05); Masson staining showed that the wound collagen in the PRP group formed quickly, had thick fibers, and was densely arranged; compared with the control group, The expression of type I collagen (1.92¡À0.09 vs. 1.18¡À0.11) and type III collagen (1.16¡À0.05 vs. 0.74¡À0.11) in cells in the PRP group was significantly increased, and the difference was statistically significant (t=22.99, P<0.01; t= 17.62, P<0.05); compared with the control group, the type I collagen in the PRP group on days 3, 6, 10, and 15 after wound formation (5.17¡À0.11 vs. 1.79¡À0.18, 6.97¡À0.09 vs. 1.96¡À0.08, 6.00¡À0.26 vs. 2.10¡À0.05, 4.95¡À0.11 vs. 3.58¡À0.09) and type III collagen (2.35¡À0.08 vs. 1.44¡À0.05, 3.08¡À0.05 vs. 1.84¡À0.06, 3.48¡À0.07 vs. 2.36¡À0.09, 4.42¡À0.07 vs. 2.77¡À0.10) were all up-regulated, and the differences were statistically significant (t=43.37~188.37, P values <0.01). Conclusion Allogeneic platelet-rich plasma can accelerate wound healing by promoting the synthesis of type ¢ñ and type ¢ó collagen. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Hou Junjie, Yang Junge, Chen Fengchao, Chen Mincai, Yan Tongtong
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