Study On The Preparation And Activity Of Antioxidant Peptides From Tilapia Skin Collagen By Three-Enzyme Method
Keywords
Three-Enzyme Method, Response Surface Method, Hydrolyzate, Antioxidant Activity, Tilapia Skin Collagen, Peptides
Abstract
In order to explore the best process for preparing tilapia skin collagen antioxidant peptide, based on the enzymatic hydrolysis of alkaline protease and trypsin, the enzymatic hydrolysis temperature, enzymatic hydrolysis time, enzymatic hydrolysis pH value and enzyme and substrate quality were used. The ratio ([E]/[S]) was used as the experimental factor, and the hydrolysis degree and DPPH free radical scavenging rate were used as response values. Response surface analysis was used to optimize the enzymatic hydrolysis conditions of Orientase 20A enzyme. The results show that the optimal process parameters are: enzymatic hydrolysis temperature of 41.74¡ãC, pH 3.97, enzymatic hydrolysis time of 6 hours, [E]/[S] of 1.5%, prediction of hydrolysis degree of enzymatic hydrolysis solution and DPPH free radical scavenging rate The values are 9.42% and 35.01% respectively, and the verification values are 9.57% and 35.21% respectively. The optimization of enzymatic hydrolysis conditions for the preparation of tilapia skin collagen antioxidant peptides by enzymatic hydrolysis of response surface is feasible and can be used for practical prediction. Antioxidant activity experiments show that the enzymatic peptide has strong ability to scavenge DPPH free radicals and ABTS+¡¤, with IC50 values of 10.78mg/mL and 8.26mg/mL respectively.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Xia Guanghua, Zhang Cheng, Xu Kehan, Shen Xuanri, Cai Jinhong
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
