Study On The Preparation Of Collagen Oligopeptides By Synergistically Hydrolyzing Pig Skin With Dual Enzymes Under Gradient Ph Conditions
Keywords
Oligopeptide, Collagen, Acid Protease, Alkaline Protease
Abstract
This article studied the feasibility of using subtilis alkaline protease (Alcalase 2.4L) and Aspergillus niger acid protease (3000U/g) to synergistically hydrolyze pig skin collagen to prepare oligopeptide hydrolysates under gradient pH conditions without the addition of alkali. sex. The effects of single-enzyme single-factor hydrolysis conditions and double-enzyme adding methods on the pig skin degradation rate and protein hydrolysis degree were investigated. On this basis, the best method for hydrolyzing pig skin under the one-time double-enzyme feeding scheme was further optimized through orthogonal experiments. Conditions: The hydrolysis temperature is 60¡ãC, the alkaline protease addition amount is 25 ¦Ìl/g protein, the acid protease addition amount is 4% (g/g), the substrate concentration is 60g/L, and the hydrolysis time is 12 hours. Under the above-mentioned optimal conditions, the degradation rate of fresh pig skin can reach 94.7%, the degree of protein hydrolysis can reach 17.7%, and the selectivity of oligopeptides with a molecular weight less than 1000 (peptides below 10 amino acids) in the hydrolyzate reaches 42.5%. . The results show that without adding alkali, the dual-enzyme synergistic hydrolysis method can significantly improve the pig skin degradation rate, especially the protein hydrolysis degree.
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Original research was done by Li Yan, Mao Shenggui, Wang Xiangsheng, Ban Yufeng, Guo Hongchen, Chen Lixing
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