Study On The Self-Assembly Kinetics Of Grass Carp Skin Collagen In Vitro
Keywords
Freshwater Fish, Collagen, Self-Assembly, Dynamics
Abstract
In vitro self-assembly is one of the important molecular behavioral characteristics of natural collagen and has a significant impact on the performance of collagen-based products. Taking grass carp skin enzymatically soluble collagen as the research object, we focused on studying the in vitro self-assembly kinetics of collagen, influencing factors, microstructure of assembled fibers and its thermal stability. The results of turbidity experiments and self-assembly degree analysis show that grass carp skin collagen has the ability to self-assemble in vitro, and its self-assembly process is affected by factors such as collagen mass concentration, pH value, ionic strength, and temperature. Under the conditions of pH 7~8, collagen mass concentration 3~5 mg/mL, system temperature 25~30¡ãC and NaCl concentration 0~200 mmol/L, collagen self-assembly process is faster and the degree of self-assembly is higher; assembly kinetic analysis The results show that at higher ionic strength (NaCl concentration 300 mmol/L) and lower assembly temperature (20 ¡æ), the collagen assembly process is manifested in three stages: nucleation, assembly and equilibrium, while at higher At the assembly temperature (25-30 ¡æ) and low ionic strength (NaCl concentration 0-200 mmol/L), the collagen assembly process is shown as: fast assembly section, low-speed assembly section and equilibrium section; collagen fiber morphological observation results show that, Grass carp skin collagen assembled fibers have typical D-period characteristics, but the D-period length value (64.6 nm) is smaller than mammalian collagen fibers (about 67 nm); differential scanning calorimetry (DSC) analysis results show that after fiber reorganization Afterwards, the thermal stability of grass carp skin collagen was significantly improved.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Yang Huan, Yang Ling, Wang Haibo, Zhao Yan, Deng Mingxia, Lu Liang
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
