Synthesis Of Sirna Loaded Collagen/Bioactive Glass Composites
Keywords
Sirna, Collagen, Bioactive Glass, Sirna, Collagen, Bioglass
Abstract
Abstract Purpose: To prepare siRNA-loaded collagen/bioglass composite materials, and preliminarily explore the feasibility of collagen/bioactive glass composite materials as both a scaffold material for inducing osteogenesis and a siRNA sustained-release system. Methods: The siRNA-loaded collagen/bioglass composite was prepared by freeze-drying method, and the scaffold material characterization, mechanical properties and degradation rate were tested. SEM and confocal microscopy were used to observe the adhesion and growth of MC3T3-E1 cells on the scaffold material, and q-PCR was used to detect the activity of siRNA released from the scaffold. Results: The siRNA-loaded collagen/bioglass composite material prepared by freeze-drying method was observed to be a sponge-like material. The bioactive glass was evenly dispersed in the collagen material, with low compressive strength and appropriate porosity. In the first week, the stent degradation rate reached 30.7%. Cells form pseudopods on the scaffold, adhere closely to the surface of the material, and the cells are closely connected. The interference efficiency of siRNA noggin released from the scaffold was 16% (P<0.05), and the difference was statistically significant. Conclusion: The siRNA-loaded collagen/bioglass composite prepared by freeze-drying method has good performance and biocompatibility. As the scaffold degrades, siRNA is released from the scaffold, and siRNA noggin maintains a certain activity. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by CHEN Yan-ling, CHEN Liang-jiao, LI Zheng-mao, LAN Ze-dong
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