The Role Of Changes In Lysine Hydroxylase 2 Activity Mediated By Tgf¦Â1/Smad3 Signaling Pathway In Collagen Deposition In Pulmonary Fibrosis
Keywords
Pulmonary Fibrosis^Lysine Hydroxylase 2^Collagen Deposition^Tgf¦Â1/Smad3 Signaling Pathway
Abstract
Abstract Purpose: To explore the effect of TGF¦Â1/Smad3 signaling pathway on changes in lysyl hydroxylase 2 (LH2) activity, as well as the role and mechanism of this change in collagen deposition in pulmonary fibrosis. Methods: The human lung fibroblast cell line HFL1 was cultured in F12 medium containing 10% fetal bovine serum. The experiment was divided into a control group, a TGF¦Â1 (10 ¦Ìg/L) stimulation group and a minoxidil (5 ¦Ìmol/L) intervention group. The same amount of culture medium was added to the control group; RNA and protein from each group were collected after 48 h, and RT- qPCR was used to detect the mRNA expression levels of PLOD2, ¦Á-SMA and COL ¢ñ, Western blot was used to detect changes in protein levels of LH2, total Smad3, phosphorylated Smad3, ¦Á-SMA, COL ¢ñ and COL ¢ô, and ELISA was used to detect hydroxylysylpyridinol in cells. (hydroxylysylpyridinoline, HP) content changes. Results: After TGF¦Â1 stimulation, the mRNA expressions of PLOD2, ¦Á-SMA and COL¢ñ were up-regulated (P<0.01); their corresponding protein levels such as LH2, phosphorylated Smad3, ¦Á-SMA, COL¢ñ and COL¢ô protein levels increased, while total Smad3 There was no change in protein levels. The mRNA and protein expression of the above genes decreased after minoxidil intervention (P<0.01). After TGF¦Â1 stimulation, ELISA detected an increase in HP content compared with the control group, and the synthesis was reduced after minoxidil intervention (P<0.05). Conclusion: The activation of the TGF¦Â1/Samd3 signaling pathway increases the expression of LH2; minoxidil reduces the expression of LH2 and the synthesis of hydroxylated collagen pyridine chains by inhibiting TGF¦Â1/Samd3 signal transduction, thereby alleviating pulmonary fibrosis. Service Recommend this article to friends Add to my bookshelf Add to citation manager E-mail Alert RSS Author related articles Shao Song Jun Haiyan Ye Xianwei Rao Shanshan Zhang Bangyan Yuan Guohang Liu Weijia Zhang Xiangyan Service author related articles For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Weijia, Ye Xianwei, Zhang Bangyan, Zhang Xiangyan, Fang Haiyan, Yuan Guohang, Shao Songjun, Rao Shanshan
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